ABSTRACT
Preeclampsia is a pregnancy-specific multisystem
disorder which is among the leading causes of maternal and infant mortality
worldwide, especially in developing countries. Its key diagnostics are
hypertension and proteinuria. This study seeks to help
develop a test for preeclampsia by documenting the color changes produced by a
bromophenol blue assay across urine samples containing varying concentrations
of albumin and check for the consistency of the colors and determine if the
color change for the set threshold values are apparent. Two different
sample-to-indicator ratios were used in the methodology, one for mild
proteinuria, 0.4g/L, and the other for severe proteinuria, 3.0g/L. It was found
out that the latter gave promising results, with the BPB assay consistently forming
a vivid blue complex with a solution containing 3.0g/L albumin. The test,
however, also yielded false positives. Th results of the experiment may be used
in the design of a home-based, inexpensive pre-diagnostic test for
preeclampsia.
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